Activation of mitochondria during embryogenesis

Mitochondria are well known as the powerhouse of cells and play a vital role in embryo development regulating energyhomeostasis.

Mitochondrial function has been studied for decades, although several aspects of mitochondrial metabolism duringdevelopment still remain unclear. Which mechanisms are responsible for the continuous increase in respiratory chain activity duringembryogenesis?

How does translational activity in the early embryo contribute to this process, and how is the translational activity inmitochondria coordinated with the translational activity in the cytoplasm?In my project, I will investigate mechanisms that underlie the increase in mitochondrial energy production during embryogenesis.Using zebrafish embryos as a model system for vertebrate development, I propose to measure mitochondrial translational activitywith de novo translation assays and assess its contribution to the assembly of new respiratory chain complexes.

Also, I will explorethe largely enigmatic mechanism of the co-regulation of cytoplasmic and mitochondrial translation.My research will provide novel insights into the universal yet poorly understood mechanisms that activate mitochondrial functionafter fertilization.

Since in the early embryo nucleus is transcriptionally inactive, my work has the potential to discover novel,transcription-independent feedback-mechanisms that balance mitochondrial and cytoplasmic translations.