Deciphering a novel interplay between RNA and chromatin

Each cell in our body contains the same genetic information, yet this information is decoded in vastly different manners, allowing different cells to acquire distinct roles, which are together needed for the proper functioning of our tissues and organs. The genetic information is stored in DNA molecules, which reside in a part of the cell called the nucleus.

In the nucleus, DNA is wrapped around a group of specialised proteins called histones, forming a DNA-protein structure known as chromatin. This protects the DNA from damage, and regulates access to the genetic information, which is crucial for determining which genes can be decoded at any given time. The packaging of chromatin therefore needs to be accurately controlled at all times, but it is not fully clear exactly his this is achieved in our cells.

Our preliminary results have revealed that an important chromatin protein named histone H1 can also bind to RNA molecules. RNA molecules are intermediate molecules which mediate the decoding of genetic information from DNA, but recent findings suggest they can also control chromatin. Our aim now is to understand how histone H1 binds to RNA, and how this binding affects the decoding of genetic information and the structure of chromatin.

To do this, we will use a variety of cutting-edge approaches to investigate how histone H1 interaction with RNA is regulated in cells. We will then reveal the impact of histone H1 binding to RNA on the decoding of genetic information. Finally, we will assess how changes in histone H1 function can control different cell behaviours such as cell-division.

Addressing these fundamental questions will greatly advance our understanding of chromatin biology and its regulation, opening up new opportunities for modulating chromatin function in biotechnology (e.g. cell reprogramming) and medicine (new therapeutic targets).