19-BBSRC-NSF/BIO genomeRxiv: a microbial whole-genome database & diagnostic marker design resource for classification, identification & data sharing

Precise identification of microorganisms that impact on society and the environment is a prerequisite for maintaining a healthy society and a healthy environment and for combating diseases, in addition to providing a sound empirical core for understanding microbiology. The DNA sequencing revolution has created the opportunity to use genome sequences of cultured and uncultured microorganisms for fast and precise identification.

However, precise identification is impossible without reference databases that precisely circumscribe classes of microorganisms with their unique characteristics, and rapid identification is impossible without fast algorithms that can handle the deluge of genome sequences being sequenced. Therefore, we will enhance our current web server to develop genomeRxiv, which will provide a database of hundreds of thousands of accurately catalogued and classified public genome sequences supplying the basic and applied research community with precise and accurate identification of unknown isolates based on their genome sequences alone.

A unique new feature will be provision of the academic, industrial, and government communities with the ability to identify, and announce, sequenced genomes without having to share sequences themselves, providing confidentiality for commercially- and ethically-sensitive organisms (e.g. production engineering strains, potential bioweapons, and benefit sharing with indigenous communities). genomeRxiv will also enable practical application of its classification scheme by providing the capability to design molecular diagnostic tools to detect specific groupings of bacteria, including high impact microorganisms, directly in the environment.

We are uniquely placed to develop genomeRxiv by leveraging the computational tools and platforms that we have already developed and by integrating them into the new web server. We will combine the highly-resolved classification framework of Life Identification Numbers (PIs Vinazter and Heath), the speed and computational efficiency of sourmash (PI Brown), and the precision and filtering of pyani (PI Pritchard), with the collaborative crowdsourcing framework of the LINbase web server (PIs Vinazter and Heath).