Human genome synthesis

The ability to make any human genome would transform hypothesis driven investigations in diverse areas of biology, genetic-archaeology and biotechnologies. However, there are currently no technologies for building human genomes.

Moreover, there is a technological void between the state of the art in the creation of synthetic genomes and what will be required to enable the synthesis of human genomes. The current challenge is therefore to bridge this void.

The goal of this proposal is to bridge the technological void between the state of the art, which has enabled the synthesis of Mbp-scale microbial genomes, and the technologies that will be required to build a human genome, which is 3 orders of magnitude larger than any synthetic genome that has been created to date.

We will establish key technologies for human genome synthesis and exemplify these technologies through the synthesis of a recoded human chromosome.