Biocatalytic cascades for the surface functionalization of lignocellulosic fibres

Lignocellulosic fibres are attractive materials that can replace traditional fossil-based products due to their unique functional properties.

Lignocellulosic biomass consist of a network of cellulose microfibrils, supported by a matrix of hemicelluloses and lignins with well-defined organization from the nano- to the macroscale.

The recalcitrant structure of lignocellulosic biomass usually requires harsh chemical processes for the production of cellulosic fibres, which result in the degradation of lignin and hemicellulose.

This project proposes a radical approach integrating enzyme technology and material sciences for the production and surface functionalization of cellulosic fibres from woody biomass, exploiting the biochemical diversity and biological role of hemicelluloses in plant cell walls.

We will apply novel alkaline-tolerant laccases for the production of holocellulose fibres preserving the hemicellulose composition and structure, which will be used as primers for the subsequent surface fibre functionalization by oxidase and transaminase cascades.

As proof-of-concept, material demonstrators will be prepared using the functionalized fibres for potential biomedical applications.

This project will provide a novel nature-inspired biocatalytic platform to functionalize lignocellulosic fibres with increased material yields and reduced chemical use, which could open new avenues for the subsequent modification of plant fibres tailored to virtually any application.