Integrated multi-omics analyses of DNA, RNA and protein using next-generation DNA sequencing

Analysis of multi-omics data, e.g.

DNA, RNA and protein data, provide the basis of large-scale data-driven biology and medicine, but is based on technologies that are difficult to integrate in a single analysis.

In this project we will develop a technology solution that enables simultaneous integrated multi-omics large-scale analyses using next generation DNA sequencing (NGS) as a single read-out technology.

The project is a collaboration between the National Genomics Infrastructure (NGI) and the Clinical Biomarkers Facility (CBF) at SciLifeLab and the Swedish biotechnology company Olink Proteomics AB and includes three parts: A.

Establishing Olink’s technology for multiplex protein analysis with an NGS readout as a service in NGI/CBF and make it available to researchers, health-care and the business sector. B.

Develop methods for simultaneous large-scale analysis of protein, DNA, and RNA targets using a single read-out technology. This project combines NGI’s sequencing expertise and Olinks experience of multiplex protein analysis using PEA. C.

Design targeted multi-omics clinical assays combining the diagnosis of DNA mutations, RNA expression levels and protein levels in a single NGS read-out.

The project will result in technologies that revolutionize our ability to generate multi-omics data from biological samples, and thereby contribute to a more complete understanding of complex biological processes and our ability to deliver on the promise of personalized health-care.