Equipment and Administrative Supplement- THE HARC CENTER 2.0: HIV ACCESSORY AND REGULATORY COMPLEXES

ADMINISTRATIVE SUPPLEMENTS - THE HARC CENTER 2.0: HIV ACCESSORY AND REGULATORY COMPLEXES PROJECT SUMMARY The primary goal of the HARC Center is the structural characterization of endogenous HIV-host protein complexes involved in HIV restriction, transcription, and latency. HARC 2.0 is supported by four Technological

Cores that serve to apply and develop innovative methods for biochemical and structural characterization of purified reconstituted complexes and dynamic endogenously purified complexes. In order to expeditiously achieve this overall mission, the HARC Center is requesting $1,499,999 total ($1,134,084 Direct Costs;

$365,915 Indirect Costs) in supplemental funding on behalf of the Proteomics and Computational Cores and Project 1 for (1) a critical piece of equipment, the Molecular Devices CellXpress.ai, an automated cell culture system equipped with a cell line incubator, a liquid handler, and a high content imager ($500,000 Total Costs;

no Indirect Costs); (2) the generation of an atlas of HIV-host protein interactions, utilizing state-of-the-art artificial intelligence tools and protein-protein interaction (PPI) mapping in primary human immune cells ($499,999 Total Costs; $305,654 Direct Costs, $194,345 Indirect Costs); and (3) the investigation of Vif

interactions with additional antiviral A3 family members ($500,000 Total Costs; $328,430 Direct Costs; $171,570 Indirect Costs).