TLT-1 intracellular function

Project Summary/Abstract TREM Like transcript (TLT-1), is a highly abundant platelet protein that mediates the earliest states of platelet activation. Inhibition of TLT-1 function is associated with bleeding in the inflammatory arena; however vascular hemostasis does not seem to be adversely affected. Our

studies have shown that TLT-1 presents itself as a potential target to control thrombosis without the introduction of bleeding. However, to pursue the therapeutic aspects of TLT-1, we must first understand the mechanisms of TLT-1 function. This project is focused on identifying the critical signaling motifs of TLT-1 function. To accomplish this goal, we have outlined two specific AIMs:

Aim 1: Characterize TLT-1 phosphorylation sites, Aim 2: Decipher the intracellular cues that regulate TLT-1 trafficking in platelets At the completion of these aims we will have a clear understanding of the importance of the platelet to regulation of edema and how we can manipulate platelet interactions to improve disease outcomes.