Shared Resource Core: Computational and technology development for spatial expression analysis.

Shared Resource Core. Computational and technology development for spatial expression analysis SUMMARY The Shared Resource Core will support the CSBC Research Center for Tumor-Immune Systems Biology by bringing the latest generation of profiling technologies to bear on all three scientific Projects. Emerging spatial

technologies are providing powerful means of interrogating the cancer microenvironment and assessing tumor- immune interactions in tissue context. The Shared Core will deliver a set of key technologies, including targeted methods for multiplexed protein and RNA transcript measurement at subcellular spatial resolution, and lower-

resolution transcriptome-scale approaches. These platforms have already been established in-house and will be further optimized for the contexts contained in this proposal. The Shared Resource Core will make these approaches available across Projects and work to optimize and customize for each tissue and biological

question, in addition to disseminating tools broadly in the community.