Deciphering the role of Sterol Metabolism in the Plant-Rhizobiome Interaction and their effects in plant performance

Rising global temperatures due to climate change are posing significant challenges for tomato production.

High temperatures adversely affect fruit yield and quality, necessitating the development of heat-tolerant tomato varieties. Cell membrane function, particularly the role of sterols, is crucial for heat tolerance.

This study aims to comprehend how modifying sterol composition can enhance tomato's resilience to high temperatures by influencing root exudate composition and shaping rhizosphere microbial communities.

To investigate the mechanisms behind high-temperature tolerance through sterol metabolism manipulation, we outline three specific objectives: (1) To investigate the sterol-mediated impact on compositional changes in root exudates under normal and high-temperature (HT) conditions. (2) To explore the link between modified sterol profile, root exudate composition, and rhizospheric microbial interactions. (3) To evaluate the impact of sterol-modified root exudates on plant performance and resource exchange under normal and HT conditions.

Our approach involves utilizing high-throughput technologies, including Gas Chromatography and Ultra-High-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry for lipid and metabolite profiling, as well as next-generation sequencing to analyze the impact of modified sterol metabolism on rhizosphere microorganisms.

Additionally, we will assess physiological and phenological parameters to determine the effect of altered sterol metabolism on plant growth in various temperature environments.

Given the critical role of Solanaceae family crops, such as tomatoes, in global and European agriculture, this research holds immense potential for enhancing food security and promoting sustainable agriculture.

Moreover, it opens doors to innovative approaches for improving plant performance, reducing reliance on agrochemicals, and fostering beneficial plant-soil-microbe interactions.