Decoding leukemia-immune cell dynamics by organism-wide cellular interaction mapping

Cellular interactions are of fundamental importance in life, orchestrating organismal development, tissue homeostasis and immunity.

In the immune system, cell-cell interactions act as central hubs for information processing and decision making that collectively determine the outcome of complex immune responses.

In leukemias, a cancer originating from immature immune cells, a multilayered network of cellular interactions between immune and leukemic cells underlies effective immune control of the cancer, immune evasion and response to immunotherapies.

However, technical limitations in studying cell-cell interactions restrict our understanding into these highly complex and dynamic processes.

In order to overcome this limitation, I propose to develop a novel interact-omics approach, capable of characterizing millions of cellular interactions across complex organ systems, entire organisms and patient cohorts.

Applying the interact-omics approach to sophisticated leukemia mouse models will enable us to dissect the dynamic cellular interaction networks between antigen-specific T cells, bystander immune cells and leukemic cells that drive anti-leukemia immunity and immune evasion.

In combination with the in vivo perturbation of cellular interactions, this will allow us to systematically decode the cellular logic of how the complex leukemia-immune interplay determines the disease course.

Additionally, by making use of leukemia patient cohorts which are either responsive or non-responsive to immunotherapy treatment, we will unravel previously unknown therapy resistance mechanisms and predict therapy response.

Together, our approach will set the basis for a comprehensive understanding of the leukemia-immune cell crosstalk underlying immune control, immune escape and therapy response, and may serve as a blueprint to fundamentally expand our insights into other biological processes driven by cellular interactions.